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Scientist Immunology

Date: Jan 26, 2022

Location: Philadelphia, PA, US, 19104

Company: Spark Therapeutics

Join the Spark Team


We were born of innovation, springing from the curiosity, imagination and dedication of remarkable scientists and healthcare visionaries. Our shared mission is to challenge the inevitability of genetic disease by discovering, developing, and delivering treatments in ways unimaginable – until now.


We don’t follow footsteps. We create the path.


Primary Duties

The Research Scientist will be a member of the Immunogenicity prediction Team within the Immunology

Department. The successful candidate’s primary responsibility will be to execute and improve

established protocols in immunology. She/He will coordinate with the lead and scientists to perform in vitro and ex vivo assays with the goal of advancing knowledge and understanding the cause and effect of gene therapy products interaction with the immune system.

The Research Scientist will be responsible for:

  • Evaluating the immunogenicity risk assessment of the candidate therapeutic vectors in preclinical development across different functional areas
  • Develops and implement novel and innovative in-vitro and ex-vivo immune assays or optimize existing cell-based assays to support preclinical and clinical studies based on regulatory guidelines.
  • Validate existing cutting-edge techniques (Single-cell analysis, RNASeq, Proteomics, flow cytometry, MSD, ELISPOT etc.) for the purpose of AAV vectors immunogenicity prediction.
  • Strategic planning, execution, and reporting of pre-clinical immunology studies; proposes novel AAV immunogenicity strategies to modulate immune responses to gene therapy.
  • Directs external collaborations with the support of a dedicated business development partner, as needed
  • Write and revise SOPs to reflect best practices and process improvement. Perform complex data analyses and prepare reports and scientific presentations.
  • Keep abreast of new scientific and technical advances reported in the literature to support expansion and improvement of immunoassay portfolio.
  • Communicate with internal and external entities, including vendors, Clinical Operations, CROs and collaborators, to ensure obligations are met with regards to custom reagent preparation, sample receipt, sample processing, and data provision


 Design, planning, execution, and reporting of immunogenicity assays  

 Data analysis, data review and preparation of study and sample results and reports 

Assay development and optimization/troubleshooting

 Write and revise SOPs, sample management

 Coordinate and communicate with vendors, CROs and collaborators


Education and Experience Requirements


  • Ph.D. in Immunology or related field required
  • 3+ years of post-graduate experience, preferably in gene therapy
  • Experience in MSD, flowcytometry, Elispot/Fluorospot, primary cell culture, qPCR, AAV etc
  • Experience in research, CRO and/or industry environment.
  • Extensive experience using analytical tools such as bioinformatics tools, GloMax, Prism and Flowjo.


Key Skills, Abilities, and Competencies

  • A strong background in immunology and AAV gene therapy is preferred
  • Expertise in development and execution of immunoassays and cell culture using primary cells or cell lines, new innovative genomics, and proteomic assays
  • Ability to work independently and as part of a team, self-motivation, adaptability, and a positive attitude
  • Excellent organizational, communication and collaborations skills
  • Keep accurate records, follow instructions, and comply with company policies



Complexity and Problem Solving

  • Develop solutions to a variety of problems of moderate scope and complexity. Refers to policies and practices for guidance.
  • Works on problems of moderate scope where analysis of situations or data requires a review of a variety of factors.  Exercises judgment within defined procedures and practices to determine appropriate action.



Please be aware that Spark mandates COVID-19 vaccination of all employees regardless of work location.  Accommodations may be made in accordance with applicable law.

Nearest Major Market: Philadelphia