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Sr Histologist

Date: Feb 2, 2019

Location: Philadelphia, PA, US

Company: Spark Therapeutics

Join the Spark Team


We were born of innovation, springing from the curiosity, imagination and dedication of remarkable scientists and healthcare visionaries. Our shared mission is to challenge the inevitability of genetic disease by discovering, developing, and delivering treatments in ways unimaginable – until now.


We don’t follow footsteps. We create the path.


This individual will be responsible for setting up the newly formed histology core at Spark Therapeutics.  The candidate will use histology-based techniques and image analysis to support all the therapeutic areas.


  • Functions include all phases of tissue preparation, including fresh or in-process specimens (paraffin and frozen), finished product for release and explanted specimens.
  • Routine H&E (Hematoxylin & Eosin) staining, special stains technique (histochemistry), immunohistochemistry, microscopic, and image analysis are required.
  • Responsible for examining tissues upon receipt and applying understanding of gross anatomy and study purpose to determine and dissect appropriate representative samples for histology testing and Pathology analysis.
  • Specimen preparation that requires a complex and thorough understanding of tissue structure, as well as a basic understanding of chemistry and physics; and how tissues react under process conditions, including decalcification, rehydration, grossing, fixation, embedding, microtomy, routine staining, special stains (histochemistry), and immunohistochemistry.
  • This requires the ability to analyze tests results to discern if false results were induced through the production process, or through the histological testing process.
  • Develop and drive histology-based assays (through external and internal collaboration) including immunohistochemistry (IHC), immunofluorescence (IF) and in-situ hybridization (ISH)
  • Perform in-house qualitative or quantitative image analysis across different tissue staining platforms
  • Summarize findings (PowerPoint presentation, research reports etc.) and communicate to peers and  management and wider Research and Clinical groups as projects evolve
  • This individual will also be responsible for maintenance, operation, qualification/validation of histology equipment, as well as training technicians and peers in histological sample handling, histochemical and immunohistochemical technique, imaging and analysis.
  • Support the Pre-Clinical Research Operations Lead in the day-to-day technical operation of the translational lab across all the therapeutic areas (Liver, CNS, Ocular, Discovery and Assay Development)
  • Performs routine assays across the different pipeline programs (e.g. ELISA, NAb assay, etc).
  • Implement new assays or optimize existing assays based on in-depth knowledge of laboratory techniques.
  • Participate in validation/qualification of bioanalytical assays such as immunoassays and cell-based assays.
  • Work with senior staff to ensure the availability of adequate laboratory materials and qualify new reagents, as needed.
  • Ensure optimal interactions across functions
  • Maintain accurate, timely and detailed records of work
  • Champion all safety aspects in the Translational Laboratories.



  • Hands-on Tissue processing, staining and image acquisition


  • Participate in investigations
  • Review and revise SOPs
  • Maintain equipment and records
  • Maintain laboratory safety procedures and compliance records


  • Prepare laboratory reagents/materials


  • Strong organizational skills.
  • Excellent writing and verbal communication skills.
  • Independently motivated, detail oriented and good problem solving ability.
  • Ability to multitask.
  • Ability to analyze and troubleshoot experiments.


  • PhD, DVM, or Masters degree in Biology, immunology, pathology, or related field highyl preferred.
  • Bachelors Degree Required.
  • Generally has 5 years of relevant experience
  • Experience in histology-based biomarker assay development and validation
  • Experience performing and troubleshooting singleplex and multiplex IHC, IF, and/or ISH assays
  • Experience in applying DNA/RNA profiling techniques in formalin-fixed tissue is preferred.
  • Excellent interpersonal and communication skills as well as the ability to work effectively as part of a cross functional team

Nearest Major Market: Philadelphia

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