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Senior Scientist, Bioanalytical Molecular assay

Date: Jan 17, 2021

Location: Philadelphia, PR, US

Company: Spark Therapeutics

Join the Spark Team


We were born of innovation, springing from the curiosity, imagination and dedication of remarkable scientists and healthcare visionaries. Our shared mission is to challenge the inevitability of genetic disease by discovering, developing, and delivering treatments in ways unimaginable – until now.


We don’t follow footsteps. We create the path.

Primary Duties


The Senior Scientist will be part of the Pre-Clinical Bioanalytics (PCB) group, supporting multiple fast-paced non-clinical studies for advancing novel gene therapy programs across all Therapeutic Areas. The position requires strong scientific and technical skills in method development and sample testing with a focus on molecular assays.  

  • Apply strong molecular biology and bioanalytical knowledge for developing nucleic acid-based bioassays to support the preclinical activities.
  • Utilize high level of technical expertise and sound judgment for troubleshooting complex molecular biology technical problems and finding appropriate solutions.
  • Expertise in DNA, RNA, and miRNA assays, including nucleic acid isolation from various tissue types.
  • Proficient with RT/ qPCR methods and modern molecular biology tools such as digital PCR for highly sensitive applications, including gene copy number.
  • Comfortable with high-throughput processing devices such as QuantStudio, QIASymphony and KingFisher to accommodate large number of sample testing.
  • Independently perform experiments and analyze data to support PCB workflow and meet strict timelines.
  • Prepare technical documentations as required.
  • Mentor junior team members as appropriate.




% of Time

Job Function and Description





  • Hands-on laboratory work, plan and perform experiments, analyze data, and record experiments using good documentation practices.
  • Technical troubleshooting and finding suitable resolutions.
  • Mentor junior team members.



  • Participate in group discussions, advance molecular bioassay capability and support PCB workflow.


  • Stay current with all required lab safety and compliance training.

Education and Experience Requirements


  • M.S./ Ph.D. in a relevant field required.
  • 3 to 5+ years laboratory experience with a focus on molecular assays.
  • Experience in research, CRO and/ or industry environment.
  • Extensive experience using analytical tools such as QuantStudio, Prism and Excel.
  • Experience with bioassays and testing of biological samples.
  • Knowledge of regulatory requirements for GLP assays is a plus.
  • Experience with gene therapy is a plus.
  • Experience with basic research is a plus.

Key Skills, Abilities, and Competencies


  • Excellent organizational and multi-tasking skills are required.
  • Excellent interpersonal, writing, and verbal communication skills are required.