Share this Job

Senior NGS Analytical Scientist

Date: Mar 29, 2021

Location: Philadelphia, PA, US

Company: Spark Therapeutics

Join the Spark Team


We were born of innovation, springing from the curiosity, imagination and dedication of remarkable scientists and healthcare visionaries. Our shared mission is to challenge the inevitability of genetic disease by discovering, developing, and delivering treatments in ways unimaginable – until now.


We don’t follow footsteps. We create the path.

We are seeking a senior NGS scientist who will be responsible for developing, optimizing, and running NGS workflows in Analytical Development.  This candidate will play a key role in executing NGS methods for viral vector-based therapies.  S/he will collaborate with other scientists to implement NGS methods for AAV gene therapy product characterization and optimization of AAV platforms.


The candidate will:

  • Develop and/or optimize NGS workflows for product characterization used in regulatory submission
  • Conduct experiments to support process development and platform development including NGS workflow automation (including DNA extraction and library synthesis)
  • Regularly interact with informatics scientists to develop and implement robust data processing pipelines
  • Be responsible for timely execution of experiments and interpretation of data
  • Keep accurate, clear, and updated notebooks and records
  • Help to maintain and troubleshoot NGS equipment
  • Provide input on design of new assays in Analytical Development and help to troubleshoot experiments
  • Author and review technical reports and SOPs
  • Collaborate with others within Process and Technology Development to support the pipeline


% of Time

Job Function and Description


Working in the lab to execute experiments and troubleshooting experiments/equipment


Design experiments, maintain notebooks, discuss and interpret data


Preparation of presentations for group meetings


Education and Experience Requirements

PhD in a relevant scientific discipline such as biological sciences, biochemistry, or analytical chemistry and 3-5 years of related experience within an established biotech or pharmaceutical company.  Or Master’s degree in a relevant scientific discipline with 5 years or more of related experience within an established biotech or pharmaceutical company.


Key Skills, Abilities, and Competencies

  • Demonstrated hands-on experience with using established and emerging NGS technologies (e.g. Illumina, PacBio, ONT, etc[VM1] …)  
  • Track record of assessing and implementing novel strategies to optimize NGS workflows
  • Familiarity with bioinformatic tools and track record of collaborating with bioinformatics scientists
  • In-depth understanding and knowledge of NGS applications to complement the hands-on experience
  • Experience with development of investigational products
  • Ability to work independently and assume ownership of experiments to drive results with minimal supervision
  • Strong analytical and problem-solving skills needed to design assays and experiments to answer complex analytical challenges
  • Must be able to work in a collaborative manner in a team-based environment
  • May provide guidance to more junior team members
  • Familiarity with qPCR and ddPCR is a plus

Complexity and Problem Solving

  • Must be able to design solutions to complex analytical problems
  • Plans, schedules, and arranges own activities to meet objectives
  • Demonstrates good judgement in selecting methods/techniques for desired outcome
  • Advanced skills in interpreting and synthesizing data and effectively communicating findings with some coaching from senior team members
  • May refer to established procedures and guidelines

Internal and External Contacts

  • May represent department as a prime contact on contracts or projects
  • Interacts with senior internal/external personnel on significant matters often requiring coordination between organizations/departments/teams
  • Interfaces with senior internal/external personnel in own area of expertise


Nearest Major Market: Philadelphia