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Senior Mass Spectrometry Analytical Scientist

Date: Jun 5, 2021

Location: Philadelphia, PA, US

Company: Spark Therapeutics

Join the Spark Team


We were born of innovation, springing from the curiosity, imagination and dedication of remarkable scientists and healthcare visionaries. Our shared mission is to challenge the inevitability of genetic disease by discovering, developing, and delivering treatments in ways unimaginable – until now.


We don’t follow footsteps. We create the path.

We are seeking a senior mass spectrometry scientist who will be responsible for developing, optimizing, and running our mass spectrometry-based proteomic workflows in Analytical Development.  This senior mass spectrometry scientist will play a key role in designing and executing LC-MS methodologies for viral vector-based therapies.  S/he will collaborate with other scientists to implement LC-MS methods for AAV gene therapy product characterization and optimization of AAV platforms.


The scientist will:

  • Maintain understanding of current regulatory expectations for host-cell protein of gene-therapy products
  • Develop and/or optimize LC-MS based workflows for host-cell protein identification and trending
  • Collaborate with informatics team to design and implement data analysis strategies for identification and semi-quantitative to quantitative protein measurements
  • Collaborate with Discovery and Process and Technology Development to support pipeline deliverables
  • Utilize automation technologies where appropriate to facilitate robust timely methods
  • Regularly interact with immunoassay team on HCP method development
  • Responsible for timely execution of experiments and interpretation of data
  • Keep accurate, clear, and updated notebooks and records
  • Help to maintain and troubleshoot LC-MS and related equipment
  • Author and review technical reports and SOPs



% of Time

Job Function and Description


Working in the lab to execute experiments and troubleshooting experiments/equipment


Design experiments, maintain notebooks, discuss and interpret data


Preparation of presentations for group meetings


Mandatory trainings and group meetings


Education and Experience Requirements

PhD in a relevant scientific discipline such as biological sciences, biochemistry, or analytical chemistry.  Post-doctoral research or 3-5 years relevant biotech or pharmaceutical company experience preferred but not required.  May have a Master’s degree in a relevant scientific discipline with 3-5 years of related experience within an established biotech or pharmaceutical company.


Key Skills, Abilities, and Competencies

  • Demonstrated hands-on experience with developing and using established and emerging MS technologies for proteomics (e.g. IMS-Q-TOF, Orbitrap, etc…) and associated acquisition software applications (e.g., MassHunter, XCalibur, Compass)
  • Hands-on experience with proteomics data processing pipelines (e.g., Mascot, Proteome Discoverer, etc…).
  • Track record of assessing and implementing novel strategies to optimize protein identification and characterization workflows
  • In-depth understanding and knowledge of MS applications to complement the hands-on experience
  • Experience with development of investigational products
  • Ability to work independently and assume ownership of experiments to drive results with minimal supervision
  • Strong analytical and problem-solving skills needed in order to design assays and experiments to answer complex analytical challenges
  • Must be able to work in a collaborative manner in a team-based environment
  • May provide guidance to more junior team members


Complexity and Problem Solving

  • Must be able to design solutions to complex analytical problems
  • Plans, schedules, and arranges own activities to meet objectives
  • Demonstrates good judgement in selecting methods/techniques for desired outcome
  • Advanced skills in interpreting and synthesizing data and effectively communicating findings with some coaching from senior team members
  • May refer to established procedures and guidelines


Internal and External Contacts

  • May represent department as a prime contact on contracts or projects
  • Interacts with senior internal/external personnel on significate matters often requiring coordination between organizations/departments/teams
  • Interfaces with senior internal/external personnel in own area of expertise


Nearest Major Market: Philadelphia