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Discovery Research Scientist

Date: Apr 28, 2021

Location: Philadelphia, PA, US

Company: Spark Therapeutics

Join the Spark Team


We were born of innovation, springing from the curiosity, imagination and dedication of remarkable scientists and healthcare visionaries. Our shared mission is to challenge the inevitability of genetic disease by discovering, developing, and delivering treatments in ways unimaginable – until now.


We don’t follow footsteps. We create the path.

Primary Duties

The Research Scientist is a member of the Discovery Research Group within the Research Department and will support laboratory research activities towards the development and testing of novel gene therapy approaches with improved safety and efficacy profiles.


He/she will be responsible for:

•            Participating in and developing discovery projects in support of current and future therapeutic programs.  

•            Leveraging advanced experimental approaches to identify cellular and molecular mechanisms regulating nucleic acid sensing, delivery, and long-term expression for gene therapy purposes.

•            Designing and supporting in vitro and in vivo studies to assess the safety and efficacy of novel gene therapy candidates.

•            Assisting with assays across the different discovery programs (e.g., ELISA, cell culture, MSD multiplex assay, qPCR, western blot).

•            Leading joint programs and collaborations with external parties.

•            Analysis and accurate documentation of assay results in an electronic lab notebook, study reports, technical documents, and other reporting formats.



% of Time           Job Function and Description

75%       Design, execute, or contribute to in vitro and in vivo studies to optimize gene delivery efficiency and advance existing and novel programs. This includes carrying out experiments on the bench, extensive literature review, and championing new experimental approaches.

20%       Generate technical reports, prepare presentation slides, document work in electronic lab notebook.   

5%         Trainings, group meetings, and administration work


Education and Experience Requirements

•            Typically has a Ph.D. in immunology or related discipline with a minimum of three years of experience in a research-based setting.

•            Strong understanding of the technical and equipment needs in a lab as well as significant hands-on experience with standard molecular and molecular/cell biology techniques such as ELISA, qPCR, cell culture, transfection, western blotting, and flow cytometry.

•            Hands-on research experience with laboratory animal models, primary cell isolation and culture.


Key Skills, Abilities, and Competencies

•            Proven ability to work independently in hands-on laboratory setting, self-motivated to learn and develop new techniques, perform multiple tasks simultaneously, keep accurate records, follow instructions, and comply with company policies.

•            Extensive training in innate immunology, signal transduction and gene expression analysis. Robust skills in molecular and cellular biology, and animal studies.

•            Experience in general and advanced lab techniques such as flow cytometry, primary cell isolation, molecular cloning, qPCR, cell culture, ELISA-based assays, reporter assays, SDS-PAGE/western blotting, gene expression assays, etc.

•            Desirable skills include practical experience with novel animal models, global data analysis (e.g., single cell sequencing, next generation sequencing).

•            Excellent communication skills (both oral and written).

•            Works with colleagues as a team player.

•            Strong organizational skills.

•            Highly adaptable and comfortable in environments with dynamic priority demands.

•            Familiar with computer software commonly used in bio/pharma industries.

•            Strong publication record is a plus.

Nearest Major Market: Philadelphia